Do Shrooms Show Up on a Drug Test?

Psychedelic mushrooms—commonly referred to as “shrooms”—contain the active compound psilocybin, a naturally occurring hallucinogen known for altering perception, mood, and cognition. Used both recreationally and in therapeutic settings, shrooms have grown in popularity over recent years, prompting more questions about how they interact with workplace policies and drug testing protocols.

So, do shrooms show up on drug test screenings? The short answer is: not usually. Most standard drug tests—like 5-panel or 10-panel screens—do not include psilocybin or its metabolites unless there’s a specific reason to test for them. However, the full answer depends on the type of test being used and who is ordering it. Certain environments, such as probation programs or the military, may conduct expanded or customized drug panels that include psilocybin detection.

This guide breaks down everything you need to know about mushroom detection on drug screens. We’ll explain what compounds are actually tested, how long shrooms stay in your system, and which types of tests—like urine, hair, and saliva—are most likely to detect them. We’ll also explore whether do psychedelic mushrooms show up on a drug test in different contexts, such as pre-employment screenings, probation conditions, and military regulations.

If you're wondering what to expect when it comes to drug testing and shroom use, you're in the right place. Let’s start by understanding what substances tests are looking for and how psilocybin shows up—if at all—on a typical panel.

What Do Shrooms Show Up As on a Drug Test?

To understand how psychedelic mushrooms may—or may not—appear on a drug test, it helps to know what your body does with them. The active compound in shrooms is psilocybin, but this substance doesn’t linger long in the body. Once ingested, psilocybin is rapidly converted into its active metabolite: psilocin. This is the actual compound responsible for the psychedelic effects of mushrooms—and it’s the one that might show up on a drug test if it's being specifically screened for.

So, what do shrooms show up as on a drug test? The answer is psilocin. However, psilocin is not included in most standard drug testing panels. It requires a specialized test that targets hallucinogenic substances, which are typically excluded from common employment drug screens. Unless there is a specific reason to test for hallucinogens, such as a legal order or employer policy, it’s unlikely that psilocybin use will be detected through routine screening.

This is why so many people ask: do shrooms show up on a drug test panel? The reality is, not all panels are created equal. The type of drug test being used—whether it’s a 5-panel, 10-panel, or something more advanced—determines the likelihood that psilocin will be on the radar. In the next section, we’ll break down each of the major drug panel types and explain which ones (if any) might test for magic mushrooms.

Do Shrooms Show Up on Standard Drug Panels?

While it’s true that psilocybin—the active ingredient in shrooms—is not commonly tested for, it’s important to look at what different types of drug panels actually include. Standard drug screenings vary in scope, and unless you’re being tested under special circumstances, most won’t check for hallucinogens like psilocybin or psilocin.

Let’s break it down by panel type to understand where psychedelic mushrooms might or might not show up:

Panel Type	Psilocybin Tested?	Notes
5-Panel	❌ No	Designed to detect THC, cocaine, opiates, amphetamines, and PCP
10-Panel	❌ Usually No	May test for additional substances like benzodiazepines and barbiturates, but rarely hallucinogens
12-Panel	⚠️ Sometimes	Some versions include LSD or other hallucinogens, but psilocybin is still not standard
DOT Test	❌ No	Federally regulated testing program does not include psychedelic mushrooms

So, do shrooms show up on a 5 panel drug test? Not at all. What about expanded versions like a 10- or 12-panel? Again, not typically—although do shrooms show up on a 10 panel drug test or do shrooms show up on a 12 panel drug test may depend on customization by an employer or court order. As for regulated screenings, do shrooms show up on a DOT drug test? No—DOT guidelines do not call for testing psilocybin or similar compounds.

The takeaway? Unless you’re subject to an advanced or custom screening, you’re unlikely to encounter a test that screens for psychedelic mushrooms in a standard drug panel. That said, some factors—like how recently you used shrooms—may still influence your risk of detection, especially in targeted testing environments. Let’s take a closer look at how long psilocybin stays in your system.

How Long Do Shrooms Stay in Your System?

Even though most drug tests don’t actively look for psilocybin or psilocin, it’s still useful to understand how long these substances remain in your body—especially if you're subject to a specialized or court-ordered screening. The duration that shrooms are detectable depends on several factors, including the type of drug test being used, your metabolism, dosage, frequency of use, and body composition.

After consumption, psilocybin is quickly broken down into psilocin, which the body processes and excretes relatively fast. For typical users, psilocin is detectable in the bloodstream or urine for only a short window. However, traces may linger longer in hair, especially if the test is specifically designed to detect psychedelic substances.

For a deeper breakdown of detection times across all substances and test types—including urine, blood, hair, and saliva—check out our complete guide to drug detection times by test type.

Test Type	Detection Window
Urine	1–3 days
Blood	Up to 24 hours
Hair	Up to 90 days (extremely rare)
Saliva	Not typically tested for psilocybin

So how long do shrooms show up on a drug test? For the most part, not long. In fact, unless you're undergoing a very specific type of screening, there’s little risk of psilocybin being detected after the first few days. That said, certain types of testing—such as those ordered for legal or professional reasons—may reach further back in time.

To better understand how those types of tests might apply to you, let’s start with one of the most thorough methods available: do shrooms show up on a hair drug test? Here’s what you need to know.

Do Shrooms Show Up on a Hair Drug Test?

Hair drug testing is one of the most comprehensive methods used to detect long-term substance use. It's especially common in court-ordered screenings, high-security job roles, and some pre-employment situations. But when it comes to psilocybin mushrooms, detection through hair tests is extremely uncommon.

So, do shrooms show up on a hair drug test? Technically, yes—they can. If a lab is specifically instructed to test for psilocybin or its metabolite psilocin, and the proper equipment and methods are in place, the substance may be detectable in hair for up to 90 days. However, this type of testing is rare because it requires advanced procedures, is costly, and is not part of standard panel testing.

Even though hair offers a long detection window, most employers and labs aren’t actively looking for psychedelic substances unless there’s a compelling reason. That means do shrooms show up on hair drug tests or do shrooms show up on a hair follicle drug test depends entirely on whether the test is tailored to include psilocybin. In most cases, it’s not.

That said, legal situations like probation or parole may involve broader drug screening protocols. If you’re under supervision or legal obligation, you may be wondering what gets tested—and how often. Let’s look at how this plays out in court-ordered or monitored environments like probation programs.

Do Shrooms Show Up on a Probation Drug Test?

When you're on probation, the rules around drug testing become stricter—and the scope of testing often expands. While psilocybin isn’t typically included in standard drug panels, court-ordered programs have the authority to use enhanced or custom tests. That means do shrooms show up on a probation drug test is a more nuanced question than it might be in a typical workplace setting.

In many jurisdictions, probation officers have the discretion to test for a broader range of substances, especially if there’s a known history of hallucinogen use. In these cases, the drug panel may be upgraded to include psilocin—the metabolite of psilocybin. Additionally, random or targeted testing can be implemented if drug use is suspected, even in the absence of prior history.

So, do shrooms show up on a drug test for probation? Not always—but they can. If your supervising agency chooses to include hallucinogens in their testing protocol, it's entirely possible that mushrooms will be on the radar. Unlike employment-related tests, probation screenings may involve stricter consequences and a lower threshold for detection.

This level of scrutiny isn’t exclusive to the court system. Other institutions with high standards of accountability—like the military—also enforce rigorous testing protocols. But does that include psychedelic mushrooms? Let’s take a closer look at how military drug testing compares.

What Real People Are Saying About Shroom Drug Tests

While scientific studies and test panels give us the formal answer, many people turn to communities like Reddit and Quora to share personal experiences with drug testing and psychedelics. Here's what some users have said:

Reddit user bruising_blue shared: “They often don't search for metabolites of LSD. I was on probation once, solely for LSD possession, went to court-mandated therapy tripping ovaries, and every test was clear. I eventually was presented a list of what they were testing for—it was about 83 different compounds. DMT, LSD, and psilocybin/psilocin were nowhere on that paper. Many hallucinogens are water soluble and also have an incredibly short metabolic half-life—even if they look for them, they will not be likely to find anything.”

Reddit user misstori1 (a medical center phlebotomist) added: “I've been through our test directory as well as our send-out test directory and [psilocin] is just not there. I've never tested anyone for it in my 7 years doing this. However, it is possible for doctors to order a urine test for psilocin through one of our reference laboratories… but it's complicated. Also, the drug urine test for psilocin requires it to be protected from light and stored at room temp, which is… odd for a urine test in general.”

While these stories don’t replace clinical data, they provide a valuable glimpse into how infrequently psilocybin is targeted in real-world testing—especially outside of custom forensic panels.

Note: These anecdotes are shared for informational purposes only. They are not medical or legal advice and should not be relied upon as such. Always consult a qualified professional regarding your specific situation.

Do Shrooms Show Up on a Military Drug Test?

The U.S. military enforces some of the most stringent drug testing protocols in the country. Active-duty service members and those in sensitive roles are regularly screened for a range of controlled substances. However, do shrooms show up on a military drug test? The answer is typically no—at least not by default.

Standard military drug panels do not include psilocybin or psilocin. These substances are not part of the federally mandated testing list that covers drugs like marijuana, cocaine, amphetamines, and opiates. That said, the military retains the authority to test for any substance if there is reasonable suspicion or if the individual is under a security clearance investigation.

So while psilocybin isn’t routinely screened, it can become a target if there's reason to believe it has been used. The military also prohibits the use of psychedelic substances entirely, regardless of whether they appear on a standard panel or not.

That said, as psilocybin products become more accessible in new forms—like chocolates, capsules, and gummies—some may wonder if the Military may incorporate more frequent testing for psilocin. Next, let's look at how those subject to DOT testing may fare.

Do Shrooms Show Up on a DOT Drug Test?

If you're subject to federally regulated drug testing—like those required by the Department of Transportation (DOT)—you may be wondering, do shrooms show up on a DOT drug test? The short answer is no. DOT drug tests follow strict federal guidelines and only screen for five specific drug categories: marijuana (THC), cocaine, amphetamines, opiates, and phencyclidine (PCP).

Psilocybin—the active compound in psychedelic mushrooms—is not included in the standard DOT testing panel. As a result, unless there's a special request or legal justification for additional screening, the use of shrooms is unlikely to be detected under DOT protocols.

However, it’s important to note that while shrooms won’t trigger a positive result, their use is still prohibited under DOT regulations. Detection or admission of use could still lead to disciplinary action or disqualification from safety-sensitive duties. Let’s explore whether do shroom gummies show up on drug test differently than traditional mushrooms.

Do Shroom Gummies Show Up on Drug Tests?

As psychedelic mushrooms gain popularity, so do their alternative forms—like chocolate bars, capsules, and gummies. These products offer a more palatable or discreet way to consume psilocybin, but they raise an important question: do shroom gummies show up on drug test results differently than traditional shrooms?

The answer depends on the active ingredient. If the gummies contain psilocybin, then everything previously discussed still applies. Once ingested, psilocybin is metabolized into psilocin, which is the compound that could potentially be detected on a drug test. Whether it comes from dried mushroom caps or a gummy bear, the body processes psilocybin the same way. Therefore, do shroom gummies show up on drug test panels? Only if the test is specifically designed to detect psilocin—which, as covered earlier, is rare in most standard screenings.

However, some gummies use different compounds entirely. For example, Wunderland’s legal mushroom gummies are made with a proprietary mushroom blend that don't contain psilocybin. In this case, you're in the clear—products like Wunderland’s are unlikely to trigger any red flags on standard or even custom drug panels.

Still, in certain high-risk or highly regulated environments, there are cases where tests are customized to go beyond standard panels. So, what about those special situations—do psychedelic mushrooms show up on a drug test in a custom screening? Let’s break that down next.

Check out this great post by Wunderland.

Can Psychedelic Mushrooms Be Detected on Custom Drug Panels?

Although most drug tests don't routinely screen for hallucinogens, there are specific situations where psychedelic substances like psilocybin are intentionally included in testing. So, do psychedelic mushrooms show up on a drug test? On a standard panel—typically no. But on a custom panel—yes, absolutely.

Certain employers, especially in high-security industries, aviation, healthcare, or law enforcement, may request specialized drug panels that go beyond the norm. The same is true for rehab centers, substance monitoring programs, or legal cases where psychedelic use is a known concern. These tests are tailored to detect substances like psilocin and LSD, but they come at a higher cost and require more sophisticated analysis.

Because these tests are expensive and less common, they're usually reserved for high-risk scenarios or individuals under close supervision. That said, if you're subject to one of these environments, it’s important to be aware of your exposure—even with substances you might assume are undetectable.

So, what’s the bottom line? Should casual or occasional users be worried about getting flagged? Let’s wrap up with a quick summary to put it all into perspective.

Conclusion: Should You Be Worried?

In most standard testing scenarios—like employment screenings or routine drug panels—psychedelic mushrooms are unlikely to show up. Substances like psilocybin and psilocin aren't part of the typical panels used in 5-, 10-, or even many 12-panel tests. However, exceptions do exist. If you're under probation, active military service, or subject to a high-security or custom drug screening, there’s a chance these substances could be detected—especially if hallucinogen testing is specifically requested.

Ultimately, while the average person has little to worry about, individuals in regulated or monitored environments should exercise caution. Understanding the nuances of what’s tested—and when—can help you make informed decisions about your health and compliance.

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Frequently Asked Questions

Do shrooms show up on a 10-panel drug test?

No, psilocybin is not typically included in a 10-panel drug test. These tests are designed to detect more commonly abused substances such as marijuana, cocaine, amphetamines, and opioids. Unless specifically modified, a 10-panel test won’t check for hallucinogens like shrooms.

Are shrooms detectable in urine?

Yes, but only for a short window. Psilocybin is metabolized into psilocin, which may be detectable in urine for about 1 to 3 days after use. Most standard urine drug tests do not screen for psilocin unless custom-ordered.

Will shrooms show up on probation or military tests?

They can. While not routinely included, both probation officers and military officials may request expanded panels that test for hallucinogens like psilocybin if there is cause or suspicion of use.

How long does psilocybin stay in your system?

Psilocybin is converted into psilocin, which is typically detectable in urine for 1–3 days, in blood for less than 24 hours, and in hair for up to 90 days in rare cases with specialized tests.

Do mushroom gummies test differently than dried mushrooms?

No. Whether consumed as dried caps or gummies, the body processes psilocybin the same way—by converting it into psilocin. The form doesn’t affect detection; only whether the test is targeting psilocin does.

Disclaimer: The information provided in this article is for educational and informational purposes only. MyDetoxShop.com does not condone or promote illegal activity, including attempting to circumvent drug testing policies. Detox products are intended for lawful use only, including general wellness, novelty, or personal privacy. Always consult a medical professional and comply with local laws and regulations.